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The essential role of bacterial D-glucose/H(+) symporters (DGTs) in glucose homeostasis has been shown by genetic and biochemical data. The crystal structure of the Escherichia coli DGT has not been reported. Here we expressed wild-type DGT (EcYfcG) and its R176L mutant in E. coli, and characterized the transporters in terms of osmotic response, substrate binding, substrate transport kinetics, and binding affinities for inhibitors. The hSLC-like EcYfcG had an apparent K(m) of 65 μM for D-glucose. The mutation of R176L reduced the K(m) to 15 μM with no significant change in the maximum uptake rate. No turnover was observed in either EcYfcG or R176L-EcYfcG when D-glucose was added to the inside of the cell; the binding to the transporter was too low to account for turnover. The substrate-bound structure, which should be the most relevant conformation for physiological function, was modeled from the crystal structure of the E. coli GlpT [Yin, Y.-C. et al. (2001) J. Biol. Chem. 276, 24816-24823] by homology modeling using a glutamate transporter as a template. The simulated substrate-bound conformation was more open than the apo one. The residue 176 of EcYfcG is located in the major substrate pathway, and side chain R176, together with its preferred rotameric states, plays a critical role in the substrate transport.1. Field of the Invention
The present invention relates to a method and system for providing teleradiology services, and more particularly, to a method and system for providing emergency teleradiology services.
2. Description of the Prior Art
Image archiving and communication system (IACS), also known as teleradiology, is a service in which an image of a patient that was acquired at one facility is sent via a telecommunications network (such as the

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