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The interaction between human serum albumin (HSA) and phyto-oestrogens (flavonoids) has been investigated with fluorescence and UV-visible spectroscopy. The interaction showed very different features depending on the type of phyto-oestrogen studied. The flavonols quercetin and myricetin and flavanone naringenin quenched the fluorescence of HSA in a static system. In contrast, the flavonol chrysin, the flavonolignans kampferol and isomenthone as well as the isoflavone equol did not interact with HSA under the same conditions. In kinetic experiments a new fluorescence-emission peak was observed at around 420 nm for all phyto-oestrogens which could be assigned to the exposed Trp-213 residue, since it was confirmed by fluorescence quenching experiments using acrylamide. The decreased fluorescence intensity observed in the presence of phyto-oestrogens can be attributed to a change in the microenvironment of Trp-213, which could be due to a change in the polarity of the protein. Static quenching constants (K(SV)), the association equilibrium constants (K(A)), thermodynamic parameters (ΔG(0), ΔH(0) and TΔS(0)) and quenching rates (k(q)) have been calculated for the interaction between HSA and each phyto-oestrogen. The results indicate that myricetin is bound to HSA with the highest affinity with a K(SV) value of 4.9×10(3)Lmol(-1), showing that it is the strongest quencher of HSA fluorescence, followed by quercetin and naringenin. The thermodynamic parameters (ΔG(0), ΔH(0), and TΔS(0)) were also calculated to evaluate
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Centre for the Arts and Humanities (formerly Centre for Arts Learning and Research and later Centre for Arts and Humanities) is a tertiary educational institution in the city of Lethbridge, Alberta. It offers degrees, diplomas and certificates to students.
Centre for the Arts and Humanities has the following degree programs:
Centre for the Arts and Humanities also offers a number of certificate programs for general studies, such as creative writing, professional development in the arts, and arts administration.
Centre for Arts Learning and Research
The centre was originally established as the Centre for Arts Learning and Research in 2000 in response to a perceived gap in post-secondary arts education in the province.
In 2007, the centre was renamed to a partnership with the University of Lethbridge, known as the Centre for Arts and Humanities. This name change came in part because of the need for the institution to access federal funding for grant applications, as the university is a federal-provincial organization. Although the name was changed, the institution maintained the former name in its code name.
In 2014, the name changed to its current name, Centre for Arts and Humanities.
List of universities and colleges in Alberta
List of universities in Canada
Category:Universities and colleges in Alberta
Category:Educational institutions established in 2000
Category:Arts organizations established in 2000
Category:LethbridgeFor example, a conventional recorder, such as a magneto-optical (MO) disk or an optical disk, is directly inserted into a recording and/or playback unit. However, the recording and/or playback unit needs to be provided on the interior side of a housing. Accordingly, the housing needs to have a large size. Further, the housing has to be kept in storage until used. Thus, a large space for storing the housing is necessary, and many operation steps for the storage and using are necessary. In addition, replacement and maintenance of the recording and